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Applied and Environmental Microbiology, December 2004, p. 7086-7092, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7086-7092.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of a Novel Dioxygenase Involved in Metabolism of o-Xylene, Toluene, and Ethylbenzene by Rhodococcus sp. Strain DK17

Dockyu Kim,1,{dagger} Jong-Chan Chae,2 Gerben J. Zylstra,2 Young-Soo Kim,3 Seong-Ki Kim,3 Myung Hee Nam,4 Young Min Kim,1 and Eungbin Kim1*

Department of Biology and Institute of Life Science and Biotechnology, Yonsei University,1 Department of Life Science, Chung-Ang University,3 Korea Basic Science Institute, Seoul, Korea,4 Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick, New Jersey2

Received 10 June 2004/ Accepted 5 August 2004

Rhodococcus sp. strain DK17 is able to grow on o-xylene, benzene, toluene, and ethylbenzene. DK17 harbors at least two megaplasmids, and the genes encoding the initial steps in alkylbenzene metabolism are present on the 330-kb pDK2. The genes encoding alkylbenzene degradation were cloned in a cosmid clone and sequenced completely to reveal 35 open reading frames (ORFs). Among the ORFs, we identified two nearly exact copies (one base difference) of genes encoding large and small subunits of an iron sulfur protein terminal oxygenase that are 6 kb apart from each other. Immediately downstream of one copy of the dioxygenase genes (akbA1a and akbA2a) is a gene encoding a dioxygenase ferredoxin component (akbA3), and downstream of the other copy (akbA1b and akbA2b) are genes putatively encoding a meta-cleavage pathway. RT-PCR experiments show that the two copies of the dioxygenase genes are operonic with the downstream putative catabolic genes and that both operons are induced by o-xylene. When expressed in Escherichia coli, AkbA1a-AkbA2a-AkbA3 transformed o-xylene into 2,3- and 3,4-dimethylphenol. These were apparently derived from an unstable o-xylene cis-3,4-dihydrodiol, which readily dehydrates. This indicates a single point of attack of the dioxygenase on the aromatic ring. In contrast, attack of AkbA1a-AkbA2a-AkbA3 on ethylbenzene resulted in the formation of two different cis-dihydrodiols resulting from an oxidation at the 2,3 and the 3,4 positions on the aromatic ring, respectively.

* Corresponding author. Mailing address: Department of Biology, Yonsei University, Seoul 120-749, Korea. Phone: 82-2-2123-2651. Fax: 82-2-312-5657. E-mail: eungbin{at}yonsei.ac.kr.

{dagger} Present address: Microbial Resources Bank, Microbial Genomics and Applications Center, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea.

Applied and Environmental Microbiology, December 2004, p. 7086-7092, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7086-7092.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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