This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tentcheva, D.
Right arrow Articles by Bergoin, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tentcheva, D.
Right arrow Articles by Bergoin, M.
Agricola
Right arrow Articles by Tentcheva, D.
Right arrow Articles by Bergoin, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2004, p. 7185-7191, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7185-7191.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prevalence and Seasonal Variations of Six Bee Viruses in Apis mellifera L. and Varroa destructor Mite Populations in France

Diana Tentcheva,{dagger} Laurent Gauthier,{dagger}* Nathalie Zappulla, Benjamin Dainat, François Cousserans, Marc Edouard Colin, and Max Bergoin

Laboratoire de Pathologie Comparée des Invertébrés UMR5087, Université Montpellier II, Montpellier, France

Received 14 April 2004/ Accepted 12 July 2004

A survey of six bee viruses on a large geographic scale was undertaken by using seemingly healthy bee colonies and the PCR technique. Samples of adult bees and pupae were collected from 36 apiaries in the spring, summer, and autumn during 2002. Varroa destructor samples were collected at the end of summer following acaricide treatment. In adult bees, during the year deformed wing virus (DWV) was found at least once in 97% of the apiaries, sacbrood virus (SBV) was found in 86% of the apiaries, chronic bee paralysis virus (CBPV) was found in 28% of the apiaries, acute bee paralysis virus (ABPV) was found in 58% of the apiaries, black queen cell virus (BQCV) was found in 86% of the apiaries, and Kashmir bee virus (KBV) was found in 17% of the apiaries. For pupae, the following frequencies were obtained: DWV, 94% of the apiaries; SBV, 80% of the apiaries; CBPV, none of the apiaries; ABPV, 23% of the apiaries; BQCV, 23% of the apiaries; and KBV, 6% of the apiaries. In Varroa samples, the following four viruses were identified: DWV (100% of the apiaries), SBV (45% of the apiaries), ABPV (36% of the apiaries), and KBV (5% of the apiaries). The latter findings support the putative role of mites in transmitting these viruses. Taken together, these data indicate that bee virus infections occur persistently in bee populations despite the lack of clinical signs, suggesting that colony disease outbreaks might result from environmental factors that lead to activation of viral replication in bees.

* Corresponding author. Mailing address: Laboratoire de Pathologie Comparée des Invertébrés UMR5087, CC 101, Université Montpellier II, 34095 Montpellier, France. Phone: 33 467 143220. Fax: 33 467 144679. E-mail: gauthier{at}univ-montp2.fr.

{dagger} D.T. and L.G. contributed equally to this work.

Applied and Environmental Microbiology, December 2004, p. 7185-7191, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7185-7191.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Highfield, A. C., El Nagar, A., Mackinder, L. C. M., Noel, L. M.-L. J., Hall, M. J., Martin, S. J., Schroeder, D. C. (2009). Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses. Appl. Environ. Microbiol. 75: 7212-7220 [Abstract] [Full Text]  
  • Patot, S., Lepetit, D., Charif, D., Varaldi, J., Fleury, F. (2009). Molecular Detection, Penetrance, and Transmission of an Inherited Virus Responsible for Behavioral Manipulation of an Insect Parasitoid. Appl. Environ. Microbiol. 75: 703-710 [Abstract] [Full Text]  
  • Ribiere, M., Lallemand, P., Iscache, A.-L., Schurr, F., Celle, O., Blanchard, P., Olivier, V., Faucon, J.-P. (2007). Spread of Infectious Chronic Bee Paralysis Virus by Honeybee (Apis mellifera L.) Feces. Appl. Environ. Microbiol. 73: 7711-7716 [Abstract] [Full Text]  
  • Yue, C., Schroder, M., Gisder, S., Genersch, E. (2007). Vertical-transmission routes for deformed wing virus of honeybees (Apis mellifera). J. Gen. Virol. 88: 2329-2336 [Abstract] [Full Text]  
  • Iqbal, J., Mueller, U. (2007). Virus infection causes specific learning deficits in honeybee foragers. Proc R Soc B 274: 1517-1521 [Abstract] [Full Text]  
  • Berenyi, O., Bakonyi, T., Derakhshifar, I., Koglberger, H., Topolska, G., Ritter, W., Pechhacker, H., Nowotny, N. (2007). Phylogenetic Analysis of Deformed Wing Virus Genotypes from Diverse Geographic Origins Indicates Recent Global Distribution of the Virus. Appl. Environ. Microbiol. 73: 3605-3611 [Abstract] [Full Text]  
  • Fujiyuki, T., Ohka, S., Takeuchi, H., Ono, M., Nomoto, A., Kubo, T. (2006). Prevalence and Phylogeny of Kakugo Virus, a Novel Insect Picorna-Like Virus That Infects the Honeybee (Apis mellifera L.), under Various Colony Conditions. J. Virol. 80: 11528-11538 [Abstract] [Full Text]  
  • Lanzi, G., de Miranda, J. R., Boniotti, M. B., Cameron, C. E., Lavazza, A., Capucci, L., Camazine, S. M., Rossi, C. (2006). Molecular and Biological Characterization of Deformed Wing Virus of Honeybees (Apis mellifera L.).. J. Virol. 80: 4998-5009 [Abstract] [Full Text]  
  • Berenyi, O., Bakonyi, T., Derakhshifar, I., Koglberger, H., Nowotny, N. (2006). Occurrence of Six Honeybee Viruses in Diseased Austrian Apiaries. Appl. Environ. Microbiol. 72: 2414-2420 [Abstract] [Full Text]  
  • Yue, C., Genersch, E. (2005). RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor). J. Gen. Virol. 86: 3419-3424 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»