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Applied and Environmental Microbiology, December 2004, p. 7303-7310, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7303-7310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular and Genetic Characterization of Propionicin F, a Bacteriocin from Propionibacterium freudenreichii

Dag Anders Brede,1* Therese Faye,1 Ola Johnsborg,1 Inger Ødegård,2 Ingolf F. Nes,1 and Helge Holo1,2

Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, Ås,1 Norwegian Dairies Association, Oslo, Norway2

Received 5 April 2004/ Accepted 30 July 2004

This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to bacteriocins. The mass of the purified bacteriocin was determined by mass spectrometry, and the N-terminal amino acid sequence was determined by Edman degradation. Sequencing of pcfA, the bacteriocin structural gene, revealed that propionicin F corresponds to a 43-amino-acid peptide in the central part of a 255-amino-acid open reading frame, suggesting that mature propionicin F is excised from the probacteriocin by N- and C-terminal proteolytic modifications. DNA sequencing and Northern blot hybridizations revealed that pcfA is cotranscribed with genes encoding a putative proline peptidase and a protein from the radical S-adenosylmethionine family. A gene encoding an ABC transporter was also identified in close proximity to the bacteriocin structural gene. The potential role of these genes in propionicin F maturation and secretion is discussed.

* Corresponding author. Mailing address: Department of Chemistry, Biotechnology and Food Science, Agricultural University of Norway, P.O. Box 5003, N-1432 Ås, Norway. Phone: 47 64 94 94 61. Fax: 47 64 94 14 65. E-mail: dag.anders.brede{at}ikbm.nlh.no.

Applied and Environmental Microbiology, December 2004, p. 7303-7310, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7303-7310.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Faye, T., Asebo, A., Salehian, Z., Langsrud, T., Nes, I. F., Brede, D. A. (2008). Construction of a Reporter Vector System for In Vivo Analysis of Promoter Activity in Propionibacterium freudenreichii. Appl. Environ. Microbiol. 74: 3615-3617 [Abstract] [Full Text]  
  • Brede, D. A., Lothe, S., Salehian, Z., Faye, T., Nes, I. F. (2007). Identification of the Propionicin F Bacteriocin Immunity Gene (pcfI) and Development of a Food-Grade Cloning System for Propionibacterium freudenreichii. Appl. Environ. Microbiol. 73: 7542-7547 [Abstract] [Full Text]  
  • Brede, D. A., Faye, T., Stierli, M. P., Dasen, G., Theiler, A., Nes, I. F., Meile, L., Holo, H. (2005). Heterologous Production of Antimicrobial Peptides in Propionibacterium freudenreichii. Appl. Environ. Microbiol. 71: 8077-8084 [Abstract] [Full Text]  


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