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Applied and Environmental Microbiology, December 2004, p. 7348-7354, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7348-7354.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Functional Analysis of the Gene Encoding Methionine-{gamma}-Lyase in Brevibacterium linens

Felix Amarita,1,2 Mireille Yvon,2 Michele Nardi,2 Emilie Chambellon,2 Jerôme Delettre,1 and Pascal Bonnarme1*

Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, Thiverval-Grignon,1 Unité de Biochimie et Structures des Protéines, Institut National de la Recherche Agronomique, Jouy en Josas, France2

Received 13 May 2004/ Accepted 3 August 2004

The enzymatic degradation of L-methionine and subsequent formation of volatile sulfur compounds (VSCs) is believed to be essential for flavor development in cheese. L-Methionine-{gamma}-lyase (MGL) can convert L-methionine to methanethiol (MTL), {alpha}-ketobutyrate, and ammonia. The mgl gene encoding MGL was cloned from the type strain Brevibacterium linens ATCC 9175 known to produce copious amounts of MTL and related VSCs. The disruption of the mgl gene, achieved in strain ATCC 9175, resulted in a 62% decrease in thiol-producing activity and a 97% decrease in total VSC production in the knockout strain. Our work shows that L-methionine degradation via {gamma}-elimination is a key step in the formation of VSCs in B. linens.

* Corresponding author. Mailing address: INRA, Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires, 78850 Thiverval-Grignon, France. Phone: 33 1 30 81 53 88. Fax: 31 1 30 81 55 97. E-mail: bonnarme{at}grignon.inra.fr.

Applied and Environmental Microbiology, December 2004, p. 7348-7354, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7348-7354.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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