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Applied and Environmental Microbiology, December 2004, p. 7520-7529, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7520-7529.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Novel Marine-Derived Actinomycete Taxa Rich in Bioactive Metabolites

Nathan A. Magarvey,^1,2 Jessica M. Keller,^1,2 Valerie Bernan,³ Martin Dworkin,¹ and David H. Sherman^1,2,4,5,6*

Department of Microbiology,¹ Biotechnology Institute, University of Minnesota, Minneapolis-St. Paul, Minnesota,² Chemical Sciences Section, Department of Natural Products Research, Wyeth Research, Pearl River, New York,³ Departments of Medicinal Chemistry,⁴ Chemistry,⁵ Microbiology & Immunology, University of Michigan, Ann Arbor, Michigan⁶

Received 15 July 2004/ Accepted 26 July 2004

A unique selective enrichment procedure has resulted in the isolation and identification of two new genera of marine-derived actinobacteria. Approximately 90% of the microorganisms cultured by using the presented method were from the prospective new genera, a result indicative of its high selectivity. In this study, 102 actinomycetes were isolated from subtidal marine sediments collected from the Bismarck Sea and the Solomon Sea off the coast of Papua New Guinea. A combination of physiological parameters, chemotaxonomic characteristics, distinguishing 16S rRNA gene sequences, and phylogenetic analysis based on 16S rRNA genes provided strong evidence for the two new genera (represented by strains of the PNG1 clade and strain UMM518) within the family Micromonosporaceae. Biological activity testing of fermentation products from the new marine-derived actinomycetes revealed that several had activities against multidrug-resistant gram-positive pathogens, malignant cells, and vaccinia virus replication.

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* Corresponding author. Mailing address: Life Sciences Institute, University of Michigan, 210 Washtenaw Ave., Ann Arbor, MI 48109-2216. Phone: (734) 615-9907. Fax: (734) 615-3641. E-mail: davidhs{at}umich.edu.

N.A.M. dedicates this article to Leo C. Vining for his contributions to the understanding of the biology and chemistry of terrestrial actinomycetes and his great skill in mentoring students interested in bacterial secondary metabolism.

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Applied and Environmental Microbiology, December 2004, p. 7520-7529, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7520-7529.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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