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Applied and Environmental Microbiology, December 2004, p. 7539-7544, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7539-7544.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Nucleotide Sequences, Genetic Organization, and Distribution of pEU30 and pEL60 from Erwinia amylovora

Gayle C. Foster,1,{dagger} Gayle C. McGhee,1 Alan L. Jones,1 and George W. Sundin1,2*

Department of Plant Pathology,1 Center for Microbial Ecology, Michigan State University, East Lansing, Michigan2

Received 19 May 2004/ Accepted 22 July 2004

ABSTRACT

The nucleotide sequences, genetic organization, and distribution of plasmids pEU30 (30,314 bp) and pEL60 (60,145 bp) from the plant pathogen Erwinia amylovora are described. The newly characterized pEU30 and pEL60 plasmids inhabited strains isolated in the western United States and Lebanon, respectively. The gene content of pEU30 resembled plasmids found in plant-associated bacteria, while that of pEL60 was most similar to IncL/M plasmids inhabiting enteric bacteria.


* Corresponding author. Mailing address: Department of Plant Pathology, Michigan State University, 103 Center for Integrated Plant Systems, East Lansing, MI 48824-1311. Phone: (517) 355-4573. Fax: (517) 353-5598. E-mail: sundin{at}msu.edu.

FOOTNOTES

{dagger} Present address: Center for Human Genomics, Wake Forest University School of Medicine, Winston-Salem, NC 27157.


Applied and Environmental Microbiology, December 2004, p. 7539-7544, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7539-7544.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




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