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SHORT REPORT

Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

Jeppe L. Nielsen,^1, Andreas Schramm,^1,2 Anne E. Bernhard,¹ Gerrit J. van den Engh,³ and David A. Stahl^1*

Department of Civil and Environmental Engineering, University of Washington,¹ Institute for Systems Biology, Seattle, Washington,³ Department of Ecological Microbiology, University of Bayreuth, Bayreuth, Germany²

Received 27 October 2003/ Accepted 22 July 2004

ABSTRACT

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

FOOTNOTES

Present address: Department of Life Sciences, Aalborg University, DK-9000 Aalborg, Denmark.