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Applied and Environmental Microbiology, December 2004, p. 7550-7554, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7550-7554.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

Jeppe L. Nielsen,1,{dagger} Andreas Schramm,1,2 Anne E. Bernhard,1 Gerrit J. van den Engh,3 and David A. Stahl1*

Department of Civil and Environmental Engineering, University of Washington,1 Institute for Systems Biology, Seattle, Washington,3 Department of Ecological Microbiology, University of Bayreuth, Bayreuth, Germany2

Received 27 October 2003/ Accepted 22 July 2004

ABSTRACT

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, University of Washington, 302 More Hall, Box 352700, Seattle, WA 98195-2700. Phone: (206) 685-3464. Fax: (206) 685-9185. E-mail: dastahl{at}u.washington.edu.

FOOTNOTES

{dagger} Present address: Department of Life Sciences, Aalborg University, DK-9000 Aalborg, Denmark.

Applied and Environmental Microbiology, December 2004, p. 7550-7554, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7550-7554.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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