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Applied and Environmental Microbiology, December 2004, p. 7561-7566, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7561-7566.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Effects of Expression of hemA and hemB Genes on Production of Porphyrin in Propionibacterium freudenreichii

Yongzhe Piao, Pornpimon Kiatpapan,{dagger} Mitsuo Yamashita,* and Yoshikatsu Murooka

Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-oka, Suita, Osaka, Japan

Received 3 March 2004/ Accepted 30 July 2004

ABSTRACT

The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to improve production of tetrapyrrole compounds, we expressed the hemA gene, which encodes {delta}-aminolevulinic acid (ALA) synthase from Rhodobacter sphaeroides, and the hemB gene, which encodes porphobilinogen (PBG) synthase from Propionibacterium freudenreichii subsp. shermanii IFO12424, either monocistronically or polycistronically in strain IFO12426. The recombinant strains accumulated larger amounts of ALA and PBG, with resultant 28- to 33-fold-higher production of porphyrinogens, such as uroporphyrinogen and coproporphyrinogen, than those observed in strain IFO12426, which harbored the shuttle vector pPK705.

* Corresponding author. Mailing address: Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-oka 2-1, Suita, Osaka 565-0871, Japan. Phone: 81-6-6879-4170. Fax: 81-6-6879-7418. E-mail: yamashita{at}bio.eng.osaka-u.ac.jp.

FOOTNOTES

{dagger} Present address: Department of Biochemistry, Faculty of Science, Rangsit University, Pantumtani 12000, Thailand.

Applied and Environmental Microbiology, December 2004, p. 7561-7566, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7561-7566.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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