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Applied and Environmental Microbiology, December 2004, p. 7578-7580, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7578-7580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Association of Escherichia coli O157:H7 with Houseflies on a Cattle Farm{dagger}

Muhammad J. Alam and Ludek Zurek*

Department of Entomology, Kansas State University, Manhattan, Kansas

Received 14 May 2004/ Accepted 10 August 2004

ABSTRACT

The ecology of Escherichia coli O157:H7 is not well understood. The aims of this study were to determine the prevalence of and characterize E. coli O157:H7 associated with houseflies (HF). Musca domestica L. HF (n = 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for E. coli O157:H7 isolation and quantification. The prevalence of E. coli O157:H7 was 2.9 and 1.4% in HF collected from feed bunks and a cattle feed storage shed, respectively. E. coli O157:H7 counts ranged from 3.0 x 101 to 1.5 x 105 CFU among the positive HF. PCR analysis of the E. coli O157:H7 isolates revealed that 90.4, 99.2, 99.2, and 100% of them (n = 125) possessed the stx1, stx2, eaeA, and fliC genes, respectively. Large populations of HF on cattle farms may play a role in the dissemination of E. coli O157:H7 among animals and to the surrounding environment.

* Corresponding author. Mailing address: Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506. Phone: (785) 532-4731. Fax: (785) 532-6232. E-mail: lzurek{at}ksu.edu.

FOOTNOTES

{dagger} Contribution no. 05-21-J from the Kansas Agricultural Experiment Station.

Applied and Environmental Microbiology, December 2004, p. 7578-7580, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7578-7580.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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