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Applied and Environmental Microbiology, February 2004, p. 1238-1241, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.1238-1241.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Overexpression of Actinobacillus succinogenes Phosphoenolpyruvate Carboxykinase on Succinate Production in Escherichia coli

Pil Kim, Maris Laivenieks, Claire Vieille, and J. Gregory Zeikus*

Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824

Received 12 August 2003/ Accepted 17 October 2003

Succinate fermentation was investigated in Escherichia coli strains overexpressing Actinobacillus succinogenes phosphoenolpyruvate carboxykinase (PEPCK). In E. coli K-12, PEPCK overexpression had no effect on succinate fermentation. In contrast, in the phosphoenolpyruvate carboxylase mutant E. coli strain K-12 ppc::kan, PEPCK overexpression increased succinate production 6.5-fold.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824-1319. Phone: (517) 353-5556. Fax: (517) 353-9334. E-mail: zeikus{at}msu.edu.

Applied and Environmental Microbiology, February 2004, p. 1238-1241, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.1238-1241.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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