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Applied and Environmental Microbiology, February 2004, p. 670-674, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.670-674.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Permanent Colonization by Lactobacillus casei Is Hindered by the Low Rate of Cell Division in Mouse Gut

Y. K. Lee,1* P. S. Ho,1 C. S. Low,1 H. Arvilommi,2 and S. Salminen3

Department of Microbiology, National University of Singapore, Singapore 117597, Republic of Singapore,1 National Public Health Institute, 20520 Turku,2 Department of Biochemistry and Food Chemistry, University of Turku, 20014 Turku, Finland3

Received 8 May 2003/ Accepted 24 October 2003

Long residence times of probiotics in the intestinal tract would prolong their potential beneficial health effects and assist colonization. This study investigated the colonization potential of Lactobacillus casei Shirota in mouse intestine by using 5 (and 6)-carboxyfluorescein diacetate, succinimidyl ester (cFDA-SE) for assessment of doubling times in different parts of the intestine. The amounts of intestinal water overlying the surfaces of the duodenum, jejunum, ileum, and colon in BALB/c mice were 34.4 ± 2.9, 58.8 ± 6.8, 21.6 ± 2.2, and 8.0 ± 1.0 mg, respectively. Based on the residual concentrations of cFDA-SE-labeled lactobacilli on intestinal mucosal surfaces, the average half times for the wash-out of lactobacilli fed were estimated at 3.98, 1.55, 1.34, and 2.48 days in the duodenum, jejunum, ileum, and colon, respectively. The average doubling times of the lactobacilli, estimated from the residual fluorescent levels of surface-adhered cells, were 4.10, 4.78, 4.56, and 5.59 days in the duodenum, jejunum, ileum, and colon, respectively. It is estimated that the lactobacilli would have to achieve an average doubling time of 1.03 to 2.04 days to colonize the various sections of the mouse intestinal tract more permanently.

* Corresponding author. Mailing address: Department of Microbiology, National University of Singapore, 5 Science Dr. 2, Singapore 117597, Republic of Singapore. Phone: 65-6874 3284. Fax: 65-6776 6872. E-mail: micleeyk{at}nus.edu.sg.

Applied and Environmental Microbiology, February 2004, p. 670-674, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.670-674.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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