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Applied and Environmental Microbiology, February 2004, p. 822-830, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.822-830.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotypic and Antibiotic Susceptibility Characteristics of a Campylobacter coli Population Isolated from Dairy Farmland in the United Kingdom

A. J. H. Leatherbarrow,1* C. A. Hart,2 R. Kemp,1 N. J. Williams,1 A. Ridley,3 M. Sharma,3 P. J. Diggle,4 E. J. Wright,1 J. Sutherst,1 and N. P. French1

DEFRA Epidemiology Fellowship Unit, University of Liverpool, Leahurst, Neston, South Wirral,1 Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool,2 Department of Bacterial Disease, Veterinary Laboratories Agency, Weybridge, Surrey,3 Department of Statistics, University of Lancaster, Lancaster, United Kingdom4

Received 14 October 2003/ Accepted 5 November 2003

Campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by Campylobacter coli. Most studies have concentrated on Campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examined the spread and relatedness of Campylobacter across a range of geographical and host boundaries. Systematic sampling of a 100-km2 area of mixed farmland in northwest England yielded 88 isolates of C. coli from a range of sample types and locations, and water was heavily represented. Screening for antibiotic resistance revealed a very low prevalence of resistance, while genotyping performed by using three methods (flaA PCR restriction fragment length polymorphism [RFLP], pulsed-field gel electrophoresis [PFGE], and fluorescent amplified fragment length polymorphism [fAFLP]) provided insights into the genomic relatedness of isolates from different locations and hosts. Isolates were classified into 23 flaA groups, 34 PFGE groups, and five major fAFLP clusters. PFGE banding analysis revealed a high level of variability and no clustering by sample type. fAFLP and flaA analyses successfully grouped the isolates by sample type. We report preliminary findings suggesting that there is a strain of C. coli which may have become adapted to survival or persistence in water and that there is a group of mainly water-derived isolates from which unusual flaA PCR fragments were recovered.

* Corresponding author. Mailing address: DEFRA Epidemiology Fellowship Unit, Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Chester High Road, Neston, South Wirral CH64 7TE, United Kingdom. Phone: 0151 794 6092. Fax: 0151 794 6005. E-mail: hleather{at}liv.ac.uk.

Applied and Environmental Microbiology, February 2004, p. 822-830, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.822-830.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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