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Applied and Environmental Microbiology, March 2004, p. 1277-1286, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1277-1286.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular Diversity of New Thermococcales Isolates from a Single Area of Hydrothermal Deep-Sea Vents as Revealed by Randomly Amplified Polymorphic DNA Fingerprinting and 16S rRNA Gene Sequence Analysis

Elodie Lepage,1,{dagger} Evelyne Marguet,2,{dagger} Claire Geslin,3 Oriane Matte-Tailliez,2 Wolfram Zillig,4 Patrick Forterre,2* and Patrick Tailliez5

Unité de Recherches Laitières et Génétique Appliquée,1 Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy-en-Josas,5 Institut de Génétique et Microbiologie, CNRS, UMR 8621, Université de Paris-Sud, Orsay,2 IUEM/UBO, Technopole Brest-Iroise, CNRS, UMR 6539, 29280 Plouzané, France,3 Max-Planck-Institut für Biochemie, Martinsried, Germany4

Received 8 August 2003/ Accepted 17 November 2003

Members of the Thermococcales are anaerobic Archaea belonging to the kingdom Euryarchaea that are studied in many laboratories as model organisms for hyperthermophiles. We describe here a molecular analysis of 86 new Thermococcales isolates collected from six different chimneys of a single hydrothermal field located in the 13°N 104°W segment of the East Pacific ridge at a depth of 2,330 m. These isolates were sorted by randomly amplified polymorphic DNA (RAPD) fingerprinting into nine groups, and nine unique RAPD profiles were obtained. One RAPD group corresponds to new isolates of Thermococcus hydrothermalis, whereas all other groups and isolates with unique profiles are different from the 22 reference strains included in this study. Analysis of 16S rRNA gene sequences of representatives of each RAPD group and unique profiles showed that one group corresponds to Pyrococcus strains, whereas all the other isolates are Thermococcus strains. We estimated that our collection may contain at least 11 new species. These putative species, isolated from a single area of hydrothermal deep-sea vents, are dispersed in the 16S rRNA tree among the reference strains previously isolated from diverse hot environments (terrestrial, shallow water, hydrothermal vents) located around the world, suggesting that there is a high degree of dispersal of Thermococcales. About one-half of our isolates contain extrachromosomal elements that could be used to search for novel replication proteins and to develop genetic tools for hyperthermophiles.

* Corresponding author. Mailing address: Institut de Génétique et Microbiologie, CNRS, UMR 8621, Université de Paris-Sud, Orsay, France. Phone: 33 01 69 15 74 89. Fax: 33 01 69 15 78 08. E-mail: forterre{at}igmors.u-psud.fr

{dagger} Elodie Lepage and Evelyne Marguet contributed equally to this work.

Applied and Environmental Microbiology, March 2004, p. 1277-1286, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1277-1286.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • White, J. R., Escobar-Paramo, P., Mongodin, E. F., Nelson, K. E., DiRuggiero, J. (2008). Extensive Genome Rearrangements and Multiple Horizontal Gene Transfers in a Population of Pyrococcus Isolates from Vulcano Island, Italy. Appl. Environ. Microbiol. 74: 6447-6451 [Abstract] [Full Text]  
  • Escobar-Paramo, P., Ghosh, S., DiRuggiero, J. (2005). Evidence for Genetic Drift in the Diversification of a Geographically Isolated Population of the Hyperthermophilic Archaeon Pyrococcus. Mol Biol Evol 22: 2297-2303 [Abstract] [Full Text]  


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