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Applied and Environmental Microbiology, March 2004, p. 1393-1396, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1393-1396.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of PCR, Electrochemical Enzyme-Linked Immunosorbent Assays, and the Standard Culture Method for Detecting Salmonella in Meat Products

Luciana Croci,1* Elisabetta Delibato,1 Giulia Volpe,2 Dario De Medici,1 and Giuseppe Palleschi2

Laboratorio Alimenti, Istituto Superiore di Sanità, 00161 Rome,1 Dipartimento di Scienze e Tecnologie Chimiche, Università "Tor Vergata," 00133 Rome, Italy2

Received 30 June 2003/ Accepted 1 December 2003

An electrochemical enzyme-linked immunosorbent assay (ELISA) coupled with flow injection analysis (ELISA-FIA) and a PCR-based method using ST11 and ST15 primers for detecting salmonellae in meat were evaluated in comparison with the International Organization for Standardization (ISO) culture method. The methods were applied to experimentally contaminated and naturally contaminated meat samples. The results showed that both ELISA-FIA and PCR allowed detection of salmonella in a product contaminated with a low number of the microorganisms (1 to 10 salmonellae/25 g) after only 5 h of incubation of preenrichment broth, and they were just as effective as the ISO method.

* Corresponding author. Mailing address: Istituto Superiore di Sanità, Laboratorio Alimenti, v. le Regina Elena 299, 00161 Rome, Italy. Phone: 39 06 49902477. Fax: 39 06 49902045. E-mail: luciana.croci{at}iss.it.

Applied and Environmental Microbiology, March 2004, p. 1393-1396, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1393-1396.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Malorny, B., Hoorfar, J. (2005). Toward Standardization of Diagnostic PCR Testing of Fecal Samples: Lessons from the Detection of Salmonellae in Pigs. J. Clin. Microbiol. 43: 3033-3037 [Full Text]  


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