This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brodhagen, M.
Right arrow Articles by Loper, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brodhagen, M.
Right arrow Articles by Loper, J. E.
Agricola
Right arrow Articles by Brodhagen, M.
Right arrow Articles by Loper, J. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2004, p. 1758-1766, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1758-1766.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Positive Autoregulation and Signaling Properties of Pyoluteorin, an Antibiotic Produced by the Biological Control Organism Pseudomonas fluorescens Pf-5

Marion Brodhagen,1,{dagger} Marcella D. Henkels,2 and Joyce E. Loper1,2*

Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331,1 Horticultural Crops Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Corvallis, Oregon 973302

Received 15 May 2003/ Accepted 27 November 2003

Pseudomonas fluorescens Pf-5, a rhizosphere bacterium, produces a suite of secondary metabolites that are toxic to seed- and root-rotting plant pathogens. Among these are the polyketide compounds pyoluteorin and 2,4-diacetylphloroglucinol. We provide evidence that pyoluteorin production is influenced by positive autoregulation. Addition of pyoluteorin to liquid cultures of Pf-5 enhanced pyoluteorin production. In addition, pyoluteorin and 2,4-diacetylphloroglucinol mutually inhibit one another's production in Pf-5. For pyoluteorin, both positive autoregulation and negative influences on production by 2,4-diacetylphloroglucinol were demonstrated at the transcriptional level by measuring activity from transcriptional fusions of an ice nucleation reporter gene (inaZ) to three separate pyoluteorin biosynthetic genes. The occurrence of pyoluteorin autoregulation in the rhizosphere was assessed on cucumber seedlings in pasteurized soil with cross-feeding experiments. In the rhizosphere, expression of a pyoluteorin biosynthesis gene by a pyoluteorin-deficient mutant of Pf-5 was enhanced by pyoluteorin produced by coinoculated cells of Pf-5. These data establish that the polyketide pyoluteorin is an autoregulatory compound and functions as a signal molecule influencing the spectrum of secondary metabolites produced by the bacterial cell.

* Corresponding author. Mailing address: Agricultural Research Service, U.S. Department of Agriculture Horticultural Crops Research Laboratory, 3420 NW Orchard Ave., Corvallis, OR 97330. Phone: (541) 738-4057. Fax: (541) 738-4025. E-mail: loperj{at}mail.science.oregonstate.edu.

{dagger} Present address: Department of Microbiology, University of Washington, Seattle, WA 98195-7242.

Applied and Environmental Microbiology, March 2004, p. 1758-1766, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1758-1766.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moynihan, J. A., Morrissey, J. P., Coppoolse, E. R., Stiekema, W. J., O'Gara, F., Boyd, E. F. (2009). Evolutionary History of the phl Gene Cluster in the Plant-Associated Bacterium Pseudomonas fluorescens. Appl. Environ. Microbiol. 75: 2122-2131 [Abstract] [Full Text]  
  • Yan, A., Huang, X., Liu, H., Dong, D., Zhang, D., Zhang, X., Xu, Y. (2007). An rhl-like quorum-sensing system negatively regulates pyoluteorin production in Pseudomonas sp. M18. Microbiology 153: 16-28 [Abstract] [Full Text]  
  • Jousset, A., Lara, E., Wall, L. G., Valverde, C. (2006). Secondary Metabolites Help Biocontrol Strain Pseudomonas fluorescens CHA0 To Escape Protozoan Grazing.. Appl. Environ. Microbiol. 72: 7083-7090 [Abstract] [Full Text]  
  • Bottiglieri, M., Keel, C. (2006). Characterization of PhlG, a Hydrolase That Specifically Degrades the Antifungal Compound 2,4-Diacetylphloroglucinol in the Biocontrol Agent Pseudomonas fluorescens CHA0. Appl. Environ. Microbiol. 72: 418-427 [Abstract] [Full Text]  
  • Brodhagen, M., Paulsen, I., Loper, J. E. (2005). Reciprocal Regulation of Pyoluteorin Production with Membrane Transporter Gene Expression in Pseudomonas fluorescens Pf-5. Appl. Environ. Microbiol. 71: 6900-6909 [Abstract] [Full Text]  
  • Compant, S., Duffy, B., Nowak, J., Clement, C., Barka, E. A. (2005). Use of Plant Growth-Promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanisms of Action, and Future Prospects. Appl. Environ. Microbiol. 71: 4951-4959 [Full Text]  
  • Parret, A. H. A., Temmerman, K., De Mot, R. (2005). Novel Lectin-Like Bacteriocins of Biocontrol Strain Pseudomonas fluorescens Pf-5. Appl. Environ. Microbiol. 71: 5197-5207 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»