This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sliwinski, M. K.
Right arrow Articles by Goodman, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sliwinski, M. K.
Right arrow Articles by Goodman, R. M.
Agricola
Right arrow Articles by Sliwinski, M. K.
Right arrow Articles by Goodman, R. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2004, p. 1821-1826, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1821-1826.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Crenarchaeal Consortia Inhabiting the Rhizosphere of Diverse Terrestrial Plants with Those in Bulk Soil in Native Environments

Marek K. Sliwinski1* and Robert M. Goodman1,2

Department of Plant Pathology and,1 Gaylord Nelson Institute for Environmental Studies, University of Wisconsin—Madison, Madison, Wisconsin 537062

Received 10 July 2003/ Accepted 2 December 2003

To explore whether the crenarchaeal consortium found in the rhizosphere is distinct from the assemblage of crenarchaeotes inhabiting bulk soil, PCR-single-stranded-conformation polymorphism (PCR-SSCP) profiles were generated for 76 plant samples collected from native environments. Divergent terrestrial plant groups including bryophytes (mosses), lycopods (club mosses), pteridophytes (ferns), gymnosperms (conifers), and angiosperms (seed plants) were collected for this study. Statistical analysis revealed significant differences between rhizosphere and bulk soil PCR-SSCP profiles (Hotelling paired T2 test, P < 0.0001), suggesting that a distinct crenarchaeal consortium is associated with plants. In general, phylotype richness increased in the rhizosphere compared to the corresponding bulk soil, although the range of this increase was variable. Examples of a major change in rhizosphere (versus bulk soil) PCR-SSCP profiles were detected for all plant groups, suggesting that crenarchaeotes form associations with phylogenetically diverse plants in native environments. In addition, examples of minor to no detectable difference were found for all terrestrial plant groups, suggesting that crenarchaeal associations with plants are mediated by environmental conditions.

* Corresponding author. Present address: Department of Botany, University of Wisconsin—Madison, 430 Lincoln Dr., Madison, WI 53706. Phone: (608) 265-7929. Fax: (608) 262-7509. E-mail: mksliwin{at}wisc.edu.

Applied and Environmental Microbiology, March 2004, p. 1821-1826, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1821-1826.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bomberg, M., Timonen, S. (2009). Effect of Tree Species and Mycorrhizal Colonization on the Archaeal Population of Boreal Forest Rhizospheres. Appl. Environ. Microbiol. 75: 308-315 [Abstract] [Full Text]  
  • Simon, H. M., Jahn, C. E., Bergerud, L. T., Sliwinski, M. K., Weimer, P. J., Willis, D. K., Goodman, R. M. (2005). Cultivation of Mesophilic Soil Crenarchaeotes in Enrichment Cultures from Plant Roots. Appl. Environ. Microbiol. 71: 4751-4760 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»