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Applied and Environmental Microbiology, March 2004, p. 1830-1832, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1830-1832.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Escherichia coli Serogroups O26 and O113 by PCR Amplification of the wzx and wzy Genes

Chitrita DebRoy,1* Elisabeth Roberts,1 James Kundrat,1 Michael A. Davis,1 Connie E. Briggs,2 and Pina M. Fratamico2

Gastroenteric Disease Center, Department of Veterinary Science, The Pennsylvania State University, University Park, Pennsylvania 16802,1 Eastern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, Pennsylvania 190382

Received 23 June 2003/ Accepted 21 November 2003

PCR-based assays for detecting enterohemorrhagic Escherichia coli serogroups O26 and O113 were developed by targeting the wzx (O-antigen flippase) and the wzy (O-antigen polymerase) genes found in the O-antigen gene cluster of each organism. The PCR assays were specific for the respective serogroups, as there was no amplification of DNA from non-O26 and non-O113 E. coli serogroups or from other bacterial genera tested. Using the PCR assays, we were able to detect the organisms in seeded apple juice inoculated at concentration levels as low as <=10 CFU/ml. The O26- and O113-specific PCR assays can potentially be used for typing E. coli O26 and O113 serogroups; these assays will offer an advantage to food and environmental microbiology laboratories in terms of identifying these non-O157 serogroups by replacing antigen-based serotyping.

* Corresponding author. Mailing address: Gastroenteric Disease Center, Wiley Lab, Wiley Lane, The Pennsylvania State University, University Park, PA 16802. Phone: (814) 863-2630. Fax: (814) 863-2167. E-mail: rcd3{at}psu.edu.

Applied and Environmental Microbiology, March 2004, p. 1830-1832, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1830-1832.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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