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Applied and Environmental Microbiology, March 2004, p. 1847-1851, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1847-1851.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Differential Expression of Desulfovibrio vulgaris Genes in Response to Cu(II) and Hg(II) Toxicity

In Seop Chang,1,{dagger} Jennifer L. Groh,1 Matthew M. Ramsey,1 Jimmy D. Ballard,1 and Lee R. Krumholz1,2*

Department of Botany and Microbiology,1 Institute for Energy and Environment, University of Oklahoma, Norman, Oklahoma 73019-02452

Received 29 August 2003/ Accepted 28 November 2003

The response of Desulfovibrio vulgaris to Cu(II) and Hg(II) was characterized. Both metals increased the lag phase, and Cu(II) reduced cell yield at concentrations as low as 50 µM. mRNA expression was analyzed using random arbitrarily primed PCR, differential display, and quantitative PCR. Both Cu(II) and Hg(II) (50 µM) caused upregulation of mRNA expression for an ATP binding protein (ORF2004) and an ATPase (ORF856) with four- to sixfold increases for Hg(II) and 1.4- to 3-fold increases with Cu(II). These results suggest that D. vulgaris uses an ATP-dependent mechanism for adapting to toxic metals in the environment.

* Corresponding author. Mailing address: Department of Botany and Microbiology, University of Oklahoma, George Lynn Cross Hall, 770 Van Vleet Oval, Norman, OK 73019. Phone: (405) 325-0437. Fax: (405) 325-7619. E-mail: krumholz{at}ou.edu.

{dagger} Present address: Water Environment and Remediation Research Center, KIST, Sungpook-ku, Seoul 136-791, Korea.

Applied and Environmental Microbiology, March 2004, p. 1847-1851, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1847-1851.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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