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Applied and Environmental Microbiology, March 2004, p. 1869-1873, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1869-1873.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

L-Alanine Auxotrophy of Lactobacillus johnsonii as Demonstrated by Physiological, Genomic, and Gene Complementation Approaches

Hengameh van der Kaaij, Frank Desiere, Beat Mollet, and Jacques-Edouard Germond*

Nestlé Research Centre, Nestec, Ltd., CH-1000 Lausanne 26, Switzerland

Received 31 October 2002/ Accepted 11 December 2003

Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.

* Corresponding author. Mailing address: Nestlé Research Centre, Nestlé, Ltd., Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland. Phone: 41-21-7858835. Fax: 41-21-7858925. E-mail: jacques-edouard.germond{at}rdls.nestle.com.

Applied and Environmental Microbiology, March 2004, p. 1869-1873, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1869-1873.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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