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 Previous Article

Applied and Environmental Microbiology, March 2004, p. 1874-1881, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1874-1881.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family

Aimin He,1 Tao Li,2 Lacy Daniels,3 Ian Fotheringham,4 and John P. N. Rosazza1*

Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy,1 Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52242,3 Schering-Plough Corporation, Union, New Jersey 07083,2 Department of Chemistry, Edinburgh University, Edinburgh, Scotland4

Received 24 July 2003/ Accepted 12 December 2003

We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had Km values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites.

* Corresponding author. Mailing address: Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy, University of Iowa, Iowa City, IA 52242-5000. Phone: (319) 335-4902. Fax: (319) 335-4901. E-mail: john-rosazza{at}uiowa.edu.

Applied and Environmental Microbiology, March 2004, p. 1874-1881, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1874-1881.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lamm, A. S., Khare, A., Conville, P., Lau, P. C. K., Bergeron, H., Rosazza, J. P. N. (2009). Nocardia iowensis sp. nov., an organism rich in biocatalytically important enzymes and nitric oxide synthase. Int. J. Syst. Evol. Microbiol. 59: 2408-2414 [Abstract] [Full Text]  
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  • Venkitasubramanian, P., Daniels, L., Rosazza, J. P. N. (2007). Reduction of Carboxylic Acids by Nocardia Aldehyde Oxidoreductase Requires a Phosphopantetheinylated Enzyme. J. Biol. Chem. 282: 478-485 [Abstract] [Full Text]  


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