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Applied and Environmental Microbiology, April 2004, p. 1895-1906, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.1895-1906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Relative Ability of Orally Administered Lactobacillus murinus To Predominate and Persist in the Porcine Gastrointestinal Tract

Gillian E. Gardiner,1 Pat G. Casey,2,3 Garrett Casey,2 P. Brendan Lynch,4 Peadar G. Lawlor,4 Colin Hill,2 Gerald F. Fitzgerald,2 Catherine Stanton,1,3 and R. Paul Ross1,3*

Dairy Products Research Center,1 Pig Production Department, Teagasc, Moorepark, Fermoy, County Cork,3 Department of Microbiology, University College Cork,2 Alimentary Pharmabiotic Center, Cork, Ireland4

Received 22 September 2003/ Accepted 21 December 2003

Five porcine-derived Lactobacillus or Pediococcus isolates administered to pigs (n = 4), either singly or as a combination at ~1010 CFU per day varied with respect to intestinal survival and persistence. Two Lactobacillus murinus strains survived best and were excreted at ~107 to 108 CFU/g of feces. In contrast, Pediococcus pentosaceus DPC6006 had the lowest fecal count at ~105 CFU/g and was excreted at a significantly lower level than both L. murinus strains. Fecal L. murinus DPC6003 counts were also significantly higher than both Lactobacillus salivarius DPC6005 and Lactobacillus pentosus DPC6004 (~106 CFU/g). The L. murinus strains persisted for at least 9 days postadministration in both the feces and the cecum. Animals fed a combination of all five strains excreted ~107 CFU of the administered strains/g, with L. murinus predominating, as determined by randomly amplified polymorphic DNA PCR. Postadministration, variation was observed between animals fed the strain combination, but in general, L. murinus DPC6002 and DPC6003 and L. pentosus DPC6004 predominated in the feces and the cecum while P. pentosaceus DPC6006 was detected only in the cecum. Fifteen days after the start of culture administration, mean fecal Enterobacteriaceae counts were significantly lower in some of the treatment groups. In addition, when mean preadministration counts were compared with those obtained after 21 days of culture administration, Enterobacteriaceae counts were reduced by ~87 to 98% in pigs fed L. salivarius DPC6005, P. pentosaceus DPC6006, L. pentosus DPC6004, and the culture mix. In conclusion, the porcine intestinal isolates have potential as probiotic feed additives for pigs, with differences in strain performance highlighting the advantages of using culture combinations.

* Corresponding author. Mailing address: Teagasc, Dairy Products Research Center, Moorepark, Fermoy, County Cork, Ireland. Phone: 353-25-42229. Fax: 353-25-42340. E-mail: pross{at}moorepark.teagasc.ie.

Applied and Environmental Microbiology, April 2004, p. 1895-1906, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.1895-1906.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Barrett, E., Hayes, M., O'Connor, P., Gardiner, G., Fitzgerald, G. F., Stanton, C., Ross, R. P., Hill, C. (2007). Salivaricin P, One of a Family of Two-Component Antilisterial Bacteriocins Produced by Intestinal Isolates of Lactobacillus salivarius. Appl. Environ. Microbiol. 73: 3719-3723 [Abstract] [Full Text]  
  • Casey, P. G., Gardiner, G. E., Casey, G., Bradshaw, B., Lawlor, P. G., Lynch, P. B., Leonard, F. C., Stanton, C., Ross, R. P., Fitzgerald, G. F., Hill, C. (2007). A Five-Strain Probiotic Combination Reduces Pathogen Shedding and Alleviates Disease Signs in Pigs Challenged with Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol. 73: 1858-1863 [Abstract] [Full Text]  
  • Corthesy, B., Gaskins, H. R., Mercenier, A. (2007). Cross-Talk between Probiotic Bacteria and the Host Immune System. J. Nutr. 137: 781S-790S [Abstract] [Full Text]  


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