This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aranda, A.
Right arrow Articles by del Olmo, M.-l.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aranda, A.
Right arrow Articles by del Olmo, M.-l.
Agricola
Right arrow Articles by Aranda, A.
Right arrow Articles by del Olmo, M.-l.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2004, p. 1913-1922, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.1913-1922.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Exposure of Saccharomyces cerevisiae to Acetaldehyde Induces Sulfur Amino Acid Metabolism and Polyamine Transporter Genes, Which Depend on Met4p and Haa1p Transcription Factors, Respectively

Agustín Aranda* and Marcel-lí del Olmo

Departament de Bioquímica i Biología Molecular, Facultat de Ciències Biològiques, Universitat de València, and Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos (IATA, CSIC), 46100 Burjassot, València, Spain

Received 8 September 2003/ Accepted 26 December 2003

Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the expression of about 400 genes. Repressed genes include many genes involved in cell cycle control, cell polarity, and the mitochondrial protein biosynthesis machinery. Increased expression is displayed in many stress response genes, as well as other families of genes, such as those encoding vitamin B1 biosynthesis machinery and proteins for aryl alcohol metabolism. The induction of genes involved in sulfur metabolism is dependent on Met4p and other well-known factors involved in the transcription of MET genes under nonrepressing conditions of sulfur metabolism. Moreover, the deletion of MET4 leads to increased acetaldehyde sensitivity. TPO genes encoding polyamine transporters are also induced by acetaldehyde; in this case, the regulation is dependent on the Haa1p transcription factor. In this paper, we discuss the connections between acetaldehyde and the processes affected by this compound in yeast cells with reference to the microarray data.

* Corresponding author. Mailing address: Departament de Bioquímica i Biología Molecular, Facultat de Ciències Biològiques, Universitat de València, Apartado de Correos 73, 46100 Burjassot, València, Spain. Phone: 34 963 90 00 22. Fax: 34 963 63 6301. E-mail: Agustin.Aranda{at}uv.es.

Applied and Environmental Microbiology, April 2004, p. 1913-1922, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.1913-1922.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Linderholm, A. L., Findleton, C. L., Kumar, G., Hong, Y., Bisson, L. F. (2008). Identification of Genes Affecting Hydrogen Sulfide Formation in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 74: 1418-1427 [Abstract] [Full Text]  
  • Maestre, O., Garcia-Martinez, T., Peinado, R. A., Mauricio, J. C. (2008). Effects of ADH2 Overexpression in Saccharomyces bayanus during Alcoholic Fermentation. Appl. Environ. Microbiol. 74: 702-707 [Abstract] [Full Text]  
  • Kaur, J., Bachhawat, A. K. (2007). Yct1p, a Novel, High-Affinity, Cysteine-Specific Transporter From the Yeast Saccharomyces cerevisiae. Genetics 176: 877-890 [Abstract] [Full Text]  
  • Zuzuarregui, A., Monteoliva, L., Gil, C., del Olmo, M.{m. d.}l. (2006). Transcriptomic and Proteomic Approach for Understanding the Molecular Basis of Adaptation of Saccharomyces cerevisiae to Wine Fermentation. Appl. Environ. Microbiol. 72: 836-847 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»