This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dopson, M.
Right arrow Articles by Bond, P. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dopson, M.
Right arrow Articles by Bond, P. L.
Agricola
Right arrow Articles by Dopson, M.
Right arrow Articles by Bond, P. L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2004, p. 2079-2088, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2079-2088.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Ferroplasma Isolates and Ferroplasma acidarmanus sp. nov., Extreme Acidophiles from Acid Mine Drainage and Industrial Bioleaching Environments

Mark Dopson,1,{dagger} Craig Baker-Austin,1 Andrew Hind,1 John P. Bowman,2 and Philip L. Bond1,3*

School of Biological Sciences,1 Centre for Ecology, Evolution and Conservation, University of East Anglia, Norwich NR4 7TJ, United Kingdom,3 School of Agricultural Science, University of Tasmania, Hobart 7001, Tasmania, Australia2

Received 23 September 2003/ Accepted 6 January 2004

Three recently isolated extremely acidophilic archaeal strains have been shown to be phylogenetically similar to Ferroplasma acidiphilum YT by 16S rRNA gene sequencing. All four Ferroplasma isolates were capable of growing chemoorganotrophically on yeast extract or a range of sugars and chemomixotrophically on ferrous iron and yeast extract or sugars, and isolate "Ferroplasma acidarmanus" Fer1T required much higher levels of organic carbon. All four isolates were facultative anaerobes, coupling chemoorganotrophic growth on yeast extract to the reduction of ferric iron. The temperature optima for the four isolates were between 35 and 42°C and the pH optima were 1.0 to 1.7, and "F. acidarmanus" Fer1T was capable of growing at pH 0. The optimum yeast extract concentration for "F. acidarmanus" Fer1T was higher than that for the other three isolates. Phenotypic results suggested that isolate "F. acidarmanus" Fer1T is of a different species than the other three strains, and 16S rRNA sequence data, DNA-DNA similarity values, and two-dimensional polyacrylamide gel electrophoresis protein profiles clearly showed that strains DR1, MT17, and YT group as a single species. "F. acidarmanus" Fer1T groups separately, and we propose the new species "F. acidarmanus" Fer1T sp. nov.

* Corresponding author. Mailing address: School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom. Phone: 44 (0)1603 593534. Fax: 44 (0)1603 592250. E-mail: phil.bond{at}uea.ac.uk.

{dagger} Present address: Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.

Applied and Environmental Microbiology, April 2004, p. 2079-2088, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2079-2088.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pugh, R. A., Honda, M., Leesley, H., Thomas, A., Lin, Y., Nilges, M. J., Cann, I. K. O., Spies, M. (2008). The Iron-containing Domain Is Essential in Rad3 Helicases for Coupling of ATP Hydrolysis to DNA Translocation and for Targeting the Helicase to the Single-stranded DNA-Double-stranded DNA Junction. J. Biol. Chem. 283: 1732-1743 [Abstract] [Full Text]  
  • Rzhepishevska, O. I., Valdes, J., Marcinkeviciene, L., Gallardo, C. A., Meskys, R., Bonnefoy, V., Holmes, D. S., Dopson, M. (2007). Regulation of a Novel Acidithiobacillus caldus Gene Cluster Involved in Metabolism of Reduced Inorganic Sulfur Compounds. Appl. Environ. Microbiol. 73: 7367-7372 [Abstract] [Full Text]  
  • Itoh, T., Yoshikawa, N., Takashina, T. (2007). Thermogymnomonas acidicola gen. nov., sp. nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan. Int. J. Syst. Evol. Microbiol. 57: 2557-2561 [Abstract] [Full Text]  
  • Allen, E. E., Tyson, G. W., Whitaker, R. J., Detter, J. C., Richardson, P. M., Banfield, J. F. (2007). Genome dynamics in a natural archaeal population. Proc. Natl. Acad. Sci. USA 104: 1883-1888 [Abstract] [Full Text]  
  • Dopson, M., Baker-Austin, C., Bond, P. L. (2005). Analysis of differential protein expression during growth states of Ferroplasma strains and insights into electron transport for iron oxidation. Microbiology 151: 4127-4137 [Abstract] [Full Text]  
  • Baker-Austin, C., Dopson, M., Wexler, M., Sawers, R. G., Bond, P. L. (2005). Molecular insight into extreme copper resistance in the extremophilic archaeon 'Ferroplasma acidarmanus' Fer1. Microbiology 151: 2637-2646 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»