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Applied and Environmental Microbiology, April 2004, p. 2318-2322, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2318-2322.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Five Membrane Filtration Methods for Recovery of Cryptosporidium and Giardia Isolates from Water Samples

T. Wohlsen,1,2* J. Bates,1 B. Gray,1 and M. Katouli2

Public Health Microbiology, Queensland Health Scientific Services, Coopers Plains,1 Faculty of Science, University of the Sunshine Coast, Maroochydore, Queensland, Australia2

Received 28 August 2003/ Accepted 6 January 2004

We evaluated the efficiency of five membrane filters for recovery of Cryptosporidium parvum oocysts and Giardia lamblia cysts. These filters included the Pall Life Sciences Envirochek (EC) standard filtration and Envirochek high-volume (EC-HV) membrane filters, the Millipore flatbed membrane filter, the Sartorius flatbed membrane filter (SMF), and the Filta-Max (FM) depth filter. Distilled and surface water samples were spiked with 10 oocysts and 10 cysts/liter. We also evaluated the recovery efficiency of the EC and EC-HV filters after a 5-s backwash postfiltration. The backwashing was not applied to the other filtration methods because of the design of the filters. Oocysts and cysts were visualized by using a fluorescent monoclonal antibody staining technique. For distilled water, the highest percent recovery for both the oocysts and cysts was obtained with the FM depth filter. However, when a 5-s backwash was applied, the EC-HV membrane filter (EC-HV-R) was superior to other filters for recovery of both oocysts (n = 53 ± 15.4 per 10 liters) and cysts (n = 59 ± 11.5 per 10 liters). This was followed by results of the FM depth filter (oocysts, 28.2 ± 8, P = 0.015; cysts, 49.8 ± 12.2, P = 0.4260), and SMF (oocysts, 16.2 ± 2.8, P = 0.0079; cysts, 35.2 ± 3, P = 0.0079). Similar results were obtained with surface water samples. Giardia cysts were recovered at higher rates than were Cryptosporidium oocysts with all five filters, regardless of backwashing. Although the time differences for completion of filtration process were not significantly different among the procedures, the EC-HV filtration with 5-s backwash was less labor demanding.

* Corresponding author. Mailing address: Public Health Microbiology, Queensland Health Scientific Services, P.O. Box 594, Archerfield, Queensland 4108, Australia. Phone: 61-7-3274-9075. Fax: 61-7-3274-9175. E-mail: Tracey_Wohlsen{at}health.qld.gov.au.

Applied and Environmental Microbiology, April 2004, p. 2318-2322, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2318-2322.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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