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Applied and Environmental Microbiology, April 2004, p. 2349-2353, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2349-2353.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Subtilosin Production by Two Bacillus subtilis Subspecies and Variance of the sbo-alb Cluster

Institut für Mikrobiologie, Johann Wolfgang Goethe-Universität, D-60439 Frankfurt am Main, Germany

Received 27 August 2003/ Accepted 6 January 2004

Eight different Bacillus subtilis strains and Bacillus atrophaeus were found to produce the bacteriocin subtilosin A. On the basis of the subtilosin gene (sbo) sequences two distinct classes of B. subtilis strains were distinguished, and they fell into the two B. subtilis subspecies (B. subtilis subsp. subtilis and B. subtilis subsp. spizizenii). The entire sequence of the subtilosin gene cluster of a B. subtilis subsp. spizizenii strain, B. subtilis ATCC 6633, was determined. This sequence exhibited a high level of homology to the sequence of the sbo-alb gene locus of B. subtilis 168. By using primer extension analysis the transcriptional start sites of sbo in B. subtilis strains ATCC 6633 and 168 were found to be 47 and 45 bp upstream of the sbo start codon, respectively. Our results provide insight into the incipient evolutionary divergence of the two B. subtilis subspecies.

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