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Applied and Environmental Microbiology, April 2004, p. 2514-2519, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2514-2519.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Growth and Sporulation of Bacillus cereus ATCC 14579 under Defined Conditions: Temporal Expression of Genes for Key Sigma Factors

Ynte P. de Vries,1,2,3* Luc M. Hornstra,1,3 Willem M. de Vos,1 and Tjakko Abee1,2

Wageningen Centre for Food Sciences,1 Laboratory of Food Microbiology, Wageningen UR,2 Agrotechnology and Food Innovations A&F, Wageningen, The Netherlands3

Received 4 August 2003/ Accepted 26 December 2003

An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.

* Corresponding author. Mailing address: Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN Wageningen, The Netherlands. Phone: 31-317-475109. Fax: 31-317-475347. E-mail: Ynte.deVries{at}wur.nl.

Applied and Environmental Microbiology, April 2004, p. 2514-2519, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2514-2519.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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