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Applied and Environmental Microbiology, April 2004, p. 2535-2539, Vol. 70, No. 4
0099-2240/04/$08.00+0 DOI: 10.1128/AEM.70.4.2535-2539.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032
Received 29 August 2003/ Accepted 21 December 2003
Bacillus subtilis spores were killed by CuCl2-ascorbic acid, chloride ions were essential for killing of spores, and spores with defective coats were killed more rapidly. CuCl2-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl2-ascorbic acid may have damage to their inner membrane.
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