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Applied and Environmental Microbiology, April 2004, p. 2540-2544, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2540-2544.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparisons of Protocols for Decontamination of Environmental Ice Samples for Biological and Molecular Examinations

S. O. Rogers,1,2* V. Theraisnathan,1 L. J. Ma,2,{dagger} Y. Zhao,2,{ddagger} G. Zhang,1 S.-G. Shin,1 J. D. Castello,2 and W. T. Starmer3

Department of Biological Sciences, Bowling Green State University, Bowling Green, Ohio 43403,1 Faculty of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, Syracuse, New York 13210,2 Department of Biology, Syracuse University, Syracuse, New York 132443

Received 5 September 2003/ Accepted 7 January 2004

Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.

* Corresponding author. Mailing address: Department of Biological Sciences, Bowling Green State University, 217 Life Sciences Bldg., Bowling Green, OH 43403. Phone: (419) 372-2333. Fax: (419) 372-2024. E-mail: srogers{at}bgnet.bgsu.edu.

{dagger} Present address: Whitehead Institute, MIT Center for Genome Research, Cambridge, MA 02141.

{ddagger} Present address: School of Medicine, Wayne State University, Detroit, MI 48201.

Applied and Environmental Microbiology, April 2004, p. 2540-2544, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2540-2544.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • D'Elia, T., Veerapaneni, R., Rogers, S. O. (2008). Isolation of Microbes from Lake Vostok Accretion Ice. Appl. Environ. Microbiol. 74: 4962-4965 [Abstract] [Full Text]  
  • Bidle, K. D., Lee, S., Marchant, D. R., Falkowski, P. G. (2007). From the Cover: Fossil genes and microbes in the oldest ice on Earth. Proc. Natl. Acad. Sci. USA 104: 13455-13460 [Abstract] [Full Text]  
  • Zhang, G., Shoham, D., Gilichinsky, D., Davydov, S., Castello, J. D., Rogers, S. O. (2006). Evidence of Influenza A Virus RNA in Siberian Lake Ice. J. Virol. 80: 12229-12235 [Abstract] [Full Text]  
  • Shafaat, H. S., Ponce, A. (2006). Applications of a Rapid Endospore Viability Assay for Monitoring UV Inactivation and Characterizing Arctic Ice Cores.. Appl. Environ. Microbiol. 72: 6808-6814 [Abstract] [Full Text]  


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