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Applied and Environmental Microbiology, April 2004, p. 2545-2550, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2545-2550.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a Reflective Solar Disinfection Pouch for Treatment of Drinking Water

D. Carey Walker,1* Soo-Voon Len,2 and Brita Sheehan1

Nestlé Product Technology Center, New Milford, Connecticut,1 Nestlé Product Technology Center, Konolfingen, Switzerland2

Received 17 July 2003/ Accepted 13 January 2004

A second-generation solar disinfection (SODIS) system (pouch) was constructed from food-grade, commercially available packaging materials selected to fully transmit and amplify the antimicrobial properties of sunlight. Depending upon the season, water source, and challenge organism, culturable bacteria were reduced between 3.5 and 5.5 log cycles. The system was also capable of reducing the background presumptive coliform population in nonsterile river water below the level of detection. Similar experiments conducted with a model virus, the F-specific RNA bacteriophage MS2, indicated that the pouch was slightly less efficient, reducing viable plaques by 3.5 log units in comparison to a 5.0 log reduction of enterotoxigenic Escherichia coli O18:H11 within the same time period. These results suggest that water of poor microbiological quality can be improved by using a freely available resource (sunlight) and a specifically designed plastic pouch constructed of food-grade packaging materials.

* Corresponding author. Present address: Chr. Hansen, Inc., 9015 Maple St., Milwaukee, WI 53214. Phone: (414) 607-5755. Fax: (414) 607-5702. E-mail: carey.walker{at}chr-hansen-us.com.

Applied and Environmental Microbiology, April 2004, p. 2545-2550, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2545-2550.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.