AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aertsen, A.
Right arrow Articles by Michiels, C. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aertsen, A.
Right arrow Articles by Michiels, C. W.
Agricola
Right arrow Articles by Aertsen, A.
Right arrow Articles by Michiels, C. W.
Applied and Environmental Microbiology, May 2004, p. 2660-2666, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2660-2666.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Heat Shock Protein-Mediated Resistance to High Hydrostatic Pressure in Escherichia coli

Abram Aertsen,1 Kristof Vanoirbeek,1 Philipp De Spiegeleer,1 Jan Sermon,1 Kristel Hauben,1 Anne Farewell,2 Thomas Nyström,2 and Chris W. Michiels1*

Laboratory of Food Microbiology, Katholieke Universiteit Leuven, Leuven, Belgium,1 Department of Cell and Molecular Biology-Microbiology, Göteborg University, Göteborg, Sweden2

Received 13 October 2003/ Accepted 11 February 2004

A random library of Escherichia coli MG1655 genomic fragments fused to a promoterless green fluorescent protein (GFP) gene was constructed and screened by differential fluorescence induction for promoters that are induced after exposure to a sublethal high hydrostatic pressure stress. This screening yielded three promoters of genes belonging to the heat shock regulon (dnaK, lon, clpPX), suggesting a role for heat shock proteins in protection against, and/or repair of, damage caused by high pressure. Several further observations provide additional support for this hypothesis: (i) the expression of rpoH, encoding the heat shock-specific sigma factor {sigma}32, was also induced by high pressure; (ii) heat shock rendered E. coli significantly more resistant to subsequent high-pressure inactivation, and this heat shock-induced pressure resistance followed the same time course as the induction of heat shock genes; (iii) basal expression levels of GFP from heat shock promoters, and expression of several heat shock proteins as determined by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of proteins extracted from pulse-labeled cells, was increased in three previously isolated pressure-resistant mutants of E. coli compared to wild-type levels.


* Corresponding author. Mailing address: Laboratory of Food Microbiology, K. U. Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee, Belgium. Phone: 32-(0)16-32 15 78. Fax: 32-(0)16-32 19 60. E-mail: Chris.Michiels{at}agr.kuleuven.ac.be.


Applied and Environmental Microbiology, May 2004, p. 2660-2666, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2660-2666.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2004 by the American Society for Microbiology. All rights reserved.