This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Silveira, M. G.
Right arrow Articles by Abee, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Silveira, M. G.
Right arrow Articles by Abee, T.
Agricola
Right arrow Articles by Silveira, M. G.
Right arrow Articles by Abee, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2004, p. 2748-2755, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2748-2755.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effect of Adaptation to Ethanol on Cytoplasmic and Membrane Protein Profiles of Oenococcus oeni

M. Graça Silveira,1 Maja Baumgärtner,2 Frank M. Rombouts,1 and Tjakko Abee1*

Laboratory of Food Microbiology, Wageningen University, 6700 EV Wageningen, The Netherlands,1 Department of Cell Biology, German Research Centre for Biotechnology, D-38124 Braunschweig, Germany2

Received 29 September 2003/ Accepted 1 February 2004

The practical application of commercial malolactic starter cultures of Oenococcus oeni surviving direct inoculation in wine requires insight into mechanisms of ethanol toxicity and of acquired ethanol tolerance in this organism. Therefore, the site-specific location of proteins involved in ethanol adaptation, including cytoplasmic, membrane-associated, and integral membrane proteins, was investigated. Ethanol triggers alterations in protein patterns of O. oeni cells stressed with 12% ethanol for 1 h and those of cells grown in the presence of 8% ethanol. Levels of inosine-5'-monophosphate dehydrogenase and phosphogluconate dehydrogenase, which generate reduced nicotinamide nucleotides, were decreased during growth in the presence of ethanol, while glutathione reductase, which consumes NADPH, was induced, suggesting that maintenance of the redox balance plays an important role in ethanol adaptation. Phosphoenolpyruvate:mannose phosphotransferase system (PTS) components of mannose PTS, including the phosphocarrier protein HPr and EIIMan, were lacking in ethanol-adapted cells, providing strong evidence that mannose PTS is absent in ethanol-adapted cells, and this represents a metabolic advantage to O. oeni cells during malolactic fermentation. In cells grown in the presence of ethanol, a large increase in the number of membrane-associated proteins was observed. Interestingly, two of these proteins, dTDT-glucose-4,6-dehydratase and D-alanine:D-alanine ligase, are known to be involved in cell wall biosynthesis. Using a proteomic approach, we provide evidence for an active ethanol adaptation response of O. oeni at the cytoplasmic and membrane protein levels.

* Corresponding author. Mailing address: Laboratory of Food Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Phone: 31317484980. Fax: 31317484978. E-mail: tjakko.abee{at}micro.fdsci.wag-ur.nl.

Applied and Environmental Microbiology, May 2004, p. 2748-2755, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2748-2755.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ruiz, L., Coute, Y., Sanchez, B., de los Reyes-Gavilan, C. G., Sanchez, J.-C., Margolles, A. (2009). The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment. Microbiology 155: 957-967 [Abstract] [Full Text]  
  • Grandvalet, C., Assad-Garcia, J. S., Chu-Ky, S., Tollot, M., Guzzo, J., Gresti, J., Tourdot-Marechal, R. (2008). Changes in membrane lipid composition in ethanol- and acid-adapted Oenococcus oeni cells: characterization of the cfa gene by heterologous complementation. Microbiology 154: 2611-2619 [Abstract] [Full Text]  
  • Chatterjee, I., Somerville, G. A., Heilmann, C., Sahl, H.-G., Maurer, H. H., Herrmann, M. (2006). Very Low Ethanol Concentrations Affect the Viability and Growth Recovery in Post-Stationary-Phase Staphylococcus aureus Populations. Appl. Environ. Microbiol. 72: 2627-2636 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»