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Applied and Environmental Microbiology, May 2004, p. 2861-2866, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2861-2866.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of Lactose- and Galactose-Utilizing Pathways from Lactic Acid Bacteria in Corynebacterium glutamicum for Production of Lysine in Whey

Eoin Barrett,1,2 Catherine Stanton,1,3 Oskar Zelder,4 Gerald Fitzgerald,2,3 and R. Paul Ross1,3*

Dairy Products Research Centre, Teagasc, Moorepark, Fermoy,1 Department of Microbiology, University College,2 Alimentary Pharmabiotic Centre,2 BASF Aktiengesellschaft, D-67056 Ludwigshafen, Germany3

Received 14 August 2003/ Accepted 15 February 2004

The genetic determinants for lactose utilization from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 and galactose utilization from Lactococcus lactis subsp. cremoris MG 1363 were heterologously expressed in the lysine-overproducing strain Corynebacterium glutamicum ATCC 21253. The C. glutamicum strains expressing the lactose permease and ß-galactosidase genes of L. delbrueckii subsp. bulgaricus exhibited ß-galactosidase activity in excess of 1,000 Miller units/ml of cells and were able to grow in medium in which lactose was the sole carbon source. Similarly, C. glutamicum strains containing the lactococcal aldose-1-epimerase, galactokinase, UDP-glucose-1-P-uridylyltransferase, and UDP-galactose-4-epimerase genes in association with the lactose permease and ß-galactosidase genes exhibited ß-galactosidase levels in excess of 730 Miller units/ml of cells and were able to grow in medium in which galactose was the sole carbon source. When grown in whey-based medium, the engineered C. glutamicum strain produced lysine at concentrations of up to 2 mg/ml, which represented a 10-fold increase over the results obtained with the lactose- and galactose-negative control, C. glutamicum 21253. Despite their increased catabolic flexibility, however, the modified corynebacteria exhibited slower growth rates and plasmid instability.

* Corresponding author. Mailing address: Dairy Quality Department, Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Ireland. Phone: 353 25 42229. Fax: 353 25 42340. E-mail: pross{at}moorepark.teagasc.ie.

Applied and Environmental Microbiology, May 2004, p. 2861-2866, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2861-2866.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rittmann, D., Lindner, S. N., Wendisch, V. F. (2008). Engineering of a Glycerol Utilization Pathway for Amino Acid Production by Corynebacterium glutamicum. Appl. Environ. Microbiol. 74: 6216-6222 [Abstract] [Full Text]  
  • Kawaguchi, H., Vertes, A. A., Okino, S., Inui, M., Yukawa, H. (2006). Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum.. Appl. Environ. Microbiol. 72: 3418-3428 [Abstract] [Full Text]  


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