This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shelobolina, E. S.
Right arrow Articles by Lovley, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shelobolina, E. S.
Right arrow Articles by Lovley, D. R.
Agricola
Right arrow Articles by Shelobolina, E. S.
Right arrow Articles by Lovley, D. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2004, p. 2959-2965, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2959-2965.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Isolation, Characterization, and U(VI)-Reducing Potential of a Facultatively Anaerobic, Acid-Resistant Bacterium from Low-pH, Nitrate- and U(VI)-Contaminated Subsurface Sediment and Description of Salmonella subterranea sp. nov.

Evgenya S. Shelobolina, Sara A. Sullivan, Kathleen R. O'Neill, Kelly P. Nevin, and Derek R. Lovley*

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003

Received 20 October 2003/ Accepted 5 February 2004

A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative motile regular rods 2.0 to 3.4 µm long and 0.7 to 0.9 µm in diameter. Strain FRCl was positive for indole production, by the methyl red test, and for ornithine decarboxylase; it was negative by the Voges-Proskauer test (for acetylmethylcarbinol production), for urea hydrolysis, for arginine dihydrolase, for lysine decarboxylase, for phenylalanine deaminase, for H2S production, and for gelatin hydrolysis. Strain FRCl was capable of using O2, NO3–, S2O32–, fumarate, and malate as terminal electron acceptors and of reducing U(VI) in the cell suspension. Analysis of the 16S rDNA sequence of the isolate indicated that this strain was 96.4% similar to Salmonella bongori and 96.3% similar to Enterobacter cloacae. Physiological and phylogenetic analyses suggested that strain FRCl belongs to the genus Salmonella and represents a new species, Salmonella subterranea sp. nov.

* Corresponding author. Mailing address: Department of Microbiology, University of Massachusetts, Morrill Science Center IVN, Amherst, MA 01003. Phone: (413) 545-9651. Fax: (413) 545-1578. E-mail: dlovley{at}microbio.umass.edu.

Applied and Environmental Microbiology, May 2004, p. 2959-2965, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2959-2965.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nilgiriwala, K. S., Alahari, A., Rao, A. S., Apte, S. K. (2008). Cloning and Overexpression of Alkaline Phosphatase PhoK from Sphingomonas sp. Strain BSAR-1 for Bioprecipitation of Uranium from Alkaline Solutions. Appl. Environ. Microbiol. 74: 5516-5523 [Abstract] [Full Text]  
  • Shelobolina, E. S., Nevin, K. P., Blakeney-Hayward, J. D., Johnsen, C. V., Plaia, T. W., Krader, P., Woodard, T., Holmes, D. E., VanPraagh, C. G., Lovley, D. R. (2007). Geobacter pickeringii sp. nov., Geobacter argillaceus sp. nov. and Pelosinus fermentans gen. nov., sp. nov., isolated from subsurface kaolin lenses. Int. J. Syst. Evol. Microbiol. 57: 126-135 [Abstract] [Full Text]  
  • Wu, Q., Sanford, R. A., Loffler, F. E. (2006). Uranium(VI) Reduction by Anaeromyxobacter dehalogenans Strain 2CP-C.. Appl. Environ. Microbiol. 72: 3608-3614 [Abstract] [Full Text]  
  • (2005). Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 55: 547-549 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»