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Applied and Environmental Microbiology, May 2004, p. 2984-2988, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2984-2988.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Polyketide Synthase from Nectria haematococca

Stephane Graziani, Christelle Vasnier, and Marie-Josee Daboussi*

Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France

Received 14 July 2003/ Accepted 18 February 2004

We identified a polyketide synthase (PKS) gene, pksN, from a strain of Nectria haematococca by complementing a mutant unable to synthesize a red perithecial pigment. pksN encodes a 2,106-amino-acid polypeptide with conserved motifs characteristic of type I PKS enzymatic domains: ß-ketoacyl synthase, acyltransferase, duplicated acyl carrier proteins, and thioesterase. The pksN product groups with the Aspergillus nidulans WA-type PKSs involved in conidial pigmentation and melanin, bikaverin, and aflatoxin biosynthetic pathways. Inactivation of pksN did not cause any visible change in fungal growth, asexual sporulation, or ascospore formation, suggesting that it is involved in a specific developmental function. We propose that pksN encodes a novel PKS required for the perithecial red pigment biosynthesis.

* Corresponding author. Mailing address: Institut de Génétique et Microbiologie, BÂtiment 400, Université Paris-Sud, F-91405 Orsay cedex, France. Phone: (33 1) 69 15 62 13. Fax: (33 1) 69 15 70 06. E-mail: daboussi{at}igmors.u-psud.fr.

Applied and Environmental Microbiology, May 2004, p. 2984-2988, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2984-2988.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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