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Applied and Environmental Microbiology, May 2004, p. 3130-3132, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3130-3132.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Expression of the Ectoine Hydroxylase Gene (thpD) from Streptomyces chrysomallus in Halomonas elongata

Julia Prabhu,1 Florian Schauwecker,1 Nicolas Grammel,1 Ullrich Keller,2* and Michael Bernhard1

ActinoDrug Pharmaceuticals GmbH, 16761 Hennigsdorf,1 Institut für Chemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, 10587 Berlin, Germany2

Received 22 October 2003/ Accepted 21 January 2004

The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.

* Corresponding author. Mailing address: Institut für Chemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstrasse 29, 10587 Berlin, Germany. Phone: 49-30-31425653. Fax: 49-30-31424783. E-mail: ullrich.keller{at}tu-berlin.de.

Applied and Environmental Microbiology, May 2004, p. 3130-3132, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3130-3132.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Kuhlmann, A. U., Bursy, J., Gimpel, S., Hoffmann, T., Bremer, E. (2008). Synthesis of the Compatible Solute Ectoine in Virgibacillus pantothenticus Is Triggered by High Salinity and Low Growth Temperature. Appl. Environ. Microbiol. 74: 4560-4563 [Abstract] [Full Text]  
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