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Functional Expression of the Ectoine Hydroxylase Gene (thpD) from Streptomyces chrysomallus in Halomonas elongata

ActinoDrug Pharmaceuticals GmbH, 16761 Hennigsdorf,¹ Institut für Chemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, 10587 Berlin, Germany²

Received 22 October 2003/ Accepted 21 January 2004

The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.

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