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Applied and Environmental Microbiology, May 2004, p. 3146-3148, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3146-3148.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Transformation of Folate-Consuming Lactobacillus gasseri into a Folate Producer

Arno Wegkamp, Marjo Starrenburg, Willem M. de Vos, Jeroen Hugenholtz,* and Wilbert Sybesma

Wageningen Centre for Food Sciences and NIZO food research, Ede, The Netherlands

Received 14 August 2003/ Accepted 20 February 2004

Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.

* Corresponding author. Mailing address: NIZO food research, P.O. Box 20, 6710 BA Ede, The Netherlands. Phone: 31-318-659540. Fax: 31-318-650400. E-mail: jeroen.hugenholtz{at}nizo.nl.

Applied and Environmental Microbiology, May 2004, p. 3146-3148, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3146-3148.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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