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Applied and Environmental Microbiology, May 2004, p. 3149-3151, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3149-3151.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of Nocardia asteroides Isolates from Different Ecological Habitats on the Basis of Repetitive Extragenic Palindromic-PCR Fingerprinting

Hideki Yamamura, Masayuki Hayakawa,* Youji Nakagawa, and Yuzuru Iimura

Division of Applied Biological Sciences, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu 400, Japan

Received 31 July 2003/ Accepted 9 December 2003

Thirteen isolates of Nocardia asteroides from both soils and aquatic samples (lake and moat sediments, as well as scum from activated sludge), together with a type strain and two known clinical isolates of this species, were characterized by repetitive extragenic palindromic-PCR fingerprinting with the BOX-A1R primer. The resulting DNA fingerprint patterns proved to be strain specific, and cluster analysis distinguished the soil isolates, the aquatic isolates, and the known strains as being in separate groups.

* Corresponding author. Mailing address: Division of Applied Biological Sciences, University of Yamanashi, Takeda-4, Kofu 400-8511, Japan. Phone: 81 (55) 220-8542. Fax: 81 (55) 220-8543. E-mail: hayakawa{at}ab11.yamanashi.ac.jp.

Applied and Environmental Microbiology, May 2004, p. 3149-3151, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3149-3151.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yamamura, H., Hayakawa, M., Nakagawa, Y., Tamura, T., Kohno, T., Komatsu, F., Iimura, Y. (2005). Nocardia takedensis sp. nov., isolated from moat sediment and scumming activated sludge. Int. J. Syst. Evol. Microbiol. 55: 433-436 [Abstract] [Full Text]  


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