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Applied and Environmental Microbiology, May 2004, p. 3167-3170, Vol. 70, No. 5
0099-2240/04/$08.00+0 DOI: 10.1128/AEM.70.5.3167-3170.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
David M. Stevenson,1 and Paul J. Weimer1,2*
Department of Bacteriology, University of WisconsinMadison,1 U.S. Dairy Forage Research Center, Agricultural Research Service, United States Department of Agriculture, Madison, Wisconsin2
Received 12 November 2003/ Accepted 2 February 2004
An
32-kDa protein (albusin B) that inhibited growth of Ruminococcus flavefaciens FD-1 was isolated from culture supernatants of Ruminococcus albus 7. Traditional cloning and gene-walking PCR techniques revealed an open reading frame (albB) encoding a protein with a predicted molecular mass of 32,168 Da. A BLAST search revealed two homologs of AlbB from the unfinished genome of R. albus 8 and moderate similarity to LlpA, a recently described 30-kDa bacteriocin from Pseudomonas sp. strain BW11M1.
Present address: Department of Anatomy, University of WisconsinMadison, Madison, Wis.
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