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Applied and Environmental Microbiology, May 2004, p. 3171-3175, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3171-3175.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex PCR with 16S rRNA Gene-Targeted Primers of Bifidobacterium spp. To Identify Sources of Fecal Pollution

X. Bonjoch, E. Ballesté, and A. R. Blanch*

Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, E-08028 Barcelona, Spain

Received 10 October 2003/ Accepted 13 January 2004

Bifidobacteria are one of the most common bacterial types found in the intestines of humans and other animals and may be used as indicators of human fecal pollution. The presence of nine human-related Bifidobacterium species was analyzed in human and animal wastewater samples of different origins by using species-specific primers based on 16S rRNA sequences. Only B. adolescentis and B. dentium were found exclusively in human sewage. A multiplex PCR approach with strain-specific primers was developed. The method showed a sensitivity threshold of 10 cells/ml. This new molecular method could provide useful information for the characterization of fecal pollution sources.

* Corresponding author. Mailing address: Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Avinguda Diagonal 645, E-08028 Barcelona, Spain. Phone: 34-934021489. Fax: 34-934110592. E-mail: ablanch{at}ub.edu.

Applied and Environmental Microbiology, May 2004, p. 3171-3175, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3171-3175.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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