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Applied and Environmental Microbiology, May 2004, p. 3176-3179, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3176-3179.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Osmotic Stress Leads to Decreased Intracellular pH of Listeria monocytogenes as Determined by Fluorescence Ratio-Imaging Microscopy

Weihuan Fang,1 Henrik Siegumfeldt,2 Birgitte Bjørn Budde,2 and Mogens Jakobsen2*

College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang 310029, People’s Republic of China,1 Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg C, Denmark2

Received 19 August 2003/ Accepted 28 January 2004

Intracellular pH (pHi) of Listeria monocytogenes was determined after exposure to NaCl or sorbitol in liquid and solid media (agar). Both compounds decreased pHi, and recovery on solid medium was impaired compared to that in liquid medium. N,N'-dicyclohexylcarbodiimide abolished pHi recovery, and lowering aw with glycerol showed no effect on pHi.

* Corresponding author. Mailing address: The Royal Veterinary and Agricultural University, Department of Dairy and Food Science, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. Phone: 45 35 28 32 16. Fax: 45 35 28 32 14. E-mail: moj{at}kvl.dk.

Applied and Environmental Microbiology, May 2004, p. 3176-3179, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3176-3179.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kastbjerg, V. G., Nielsen, D. S., Arneborg, N., Gram, L. (2009). Response of Listeria monocytogenes to Disinfection Stress at the Single-Cell and Population Levels as Monitored by Intracellular pH Measurements and Viable-Cell Counts. Appl. Environ. Microbiol. 75: 4550-4556 [Abstract] [Full Text]  


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