This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bishop, R.
Right arrow Articles by Odenyo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bishop, R.
Right arrow Articles by Odenyo, A.
Agricola
Right arrow Articles by Bishop, R.
Right arrow Articles by Odenyo, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2004, p. 3180-3182, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3180-3182.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Specific PCR Assay for a Tannin-Tolerant Selenomonas ruminantium Isolate, Derived from Helicase Coding Sequences{dagger}

Richard Bishop,* Moses Obura, David Odongo, and Agnes Odenyo{ddagger}

The International Livestock Research Institute, Nairobi, Kenya, and Addis Ababa, Ethiopia

Received 21 January 2004/ Accepted 28 January 2004

Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.

* Corresponding author. Mailing address: ILRI, Old Naivasha Rd., Kabete, P.O. Box 30709, Nairobi, Kenya. Phone: 254 (020) 630 743, ext. 4106. Fax: 254 (020) 631 499. E-mail: R.Bishop{at}cgiar.org.

{dagger} This is ILRI publication number 200374.

{ddagger} Present address: The International Centre for Research on Agroforestry, Nairobi, Kenya.

Applied and Environmental Microbiology, May 2004, p. 3180-3182, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3180-3182.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»