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Applied and Environmental Microbiology, June 2004, p. 3292-3297, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3292-3297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Novel Expression System for Large-Scale Production and Purification of Recombinant Class IIa Bacteriocins and Its Application to Piscicolin 126

Gerard M. Gibbs,1,{dagger} Barrie E. Davidson,1 and Alan J. Hillier2,3*

Department of Biochemistry and Molecular Biology,1 School of Agriculture and Food Systems, University of Melbourne, Parkville, Victoria 3052,2 Food Science Australia, Werribee, Victoria 3030, Australia3

Received 28 August 2003/ Accepted 23 February 2004

Piscicolin 126 is a class IIa bacteriocin isolated from Carnobacterium piscicola JG126 that exhibits strong activity against Listeria monocytogenes. The gene encoding mature piscicolin 126 (m-pisA) was cloned into an Escherichia coli expression system and expressed as a thioredoxin-piscicolin 126 fusion protein that was purified by affinity chromatography. Purified recombinant piscicolin 126 was obtained after CNBr cleavage of the fusion protein followed by reversed-phase chromatography. Recombinant piscicolin 126 contained a single disulfide bond and had a mass identical to that of native piscicolin 126. This novel bacteriocin expression system generated approximately 26 mg of purified bacteriocin from 1 liter of E. coli culture. The purified recombinant piscicolin 126 acted by disruption of the bacterial cell membrane.

* Corresponding author. Mailing address: Food Science Australia, Private Bag 16, Werribee, Vic. 3030, Australia. Phone: 613 9731 3268. Fax: 613 9731 3206. E-mail: alan.hillier{at}foodscience.afisc.csiro.au.

{dagger} Present address: Monash Institute of Reproduction and Development, Monash University, Clayton, Victoria 3168, Australia.

Applied and Environmental Microbiology, June 2004, p. 3292-3297, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3292-3297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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