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Applied and Environmental Microbiology, June 2004, p. 3338-3345, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3338-3345.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Establishment of a Pure Culture of the Hitherto Uncultured Unicellular Cyanobacterium Aphanothece sacrum, and Phylogenetic Position of the Organism

Tsuneo Fujishiro,1* Takahira Ogawa,2 Masayoshi Matsuoka,2 Kazuhiro Nagahama,2 Yasunobu Takeshima,1 and Hideaki Hagiwara1

Hagiwara Institute of Health, Kasai, Hyogo 679-0105,1 Department of Applied Microbial Technology, Faculty of Engineering, Sojo University, Kumamoto, Kumamoto 860-0082, Japan2

Received 2 December 2003/ Accepted 29 February 2004

Aphanothece sacrum, an edible freshwater unicellular cyanobacterium, was isolated by using novel synthetic media (designated AST and AST-5xNP). The media were designed on the basis of the ratio of inorganic elements contained in A. sacrum cells cultured in a natural pond. The isolated strain exhibits unicellular rod-shaped cells ~6 µm in length that are scattered in an exopolysaccharide matrix, a feature similar to that of natural A. sacrum. DNA analysis of the isolated strain revealed that it carried two ferredoxin genes whose deduced amino acid sequences were almost identical to previously published sequences of ferredoxins from natural A. sacrum. Analysis of the 16S rRNA gene and ferredoxin genes revealed that A. sacrum occupies a phylogenetically unique position among the cyanobacteria.

* Corresponding author. Mailing address: Hagiwara Institute of Health, 1173 Asazuma-cho, Kasai, Hyogo 679-0105, Japan. Phone: 81-790-47-1581. Fax: 81-790-47-1585. E-mail: fujishiro{at}hih.gr.jp.

Applied and Environmental Microbiology, June 2004, p. 3338-3345, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3338-3345.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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