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Applied and Environmental Microbiology, June 2004, p. 3352-3359, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3352-3359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Expression of Functional Recombinant Mussel Adhesive Protein Mgfp-5 in Escherichia coli

Dong Soo Hwang,1,2 Hyo Jin Yoo,2 Jong Hyub Jun,3,4 Won Kyu Moon,3,4 and Hyung Joon Cha1,2*

Division of Molecular and Life Sciences,1 Department of Chemical Engineering,2 Division of Mechanical and Industrial Engineering,3 Department of Mechanical Engineering Pohang University of Science and Technology, Pohang 790-784, Korea4

Received 25 December 2003/ Accepted 29 February 2004

Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the first time. Using this cDNA, we produced a recombinant Mgfp-5 fused with a hexahistidine affinity ligand, which was expressed in a soluble form in Escherichia coli and was highly purified using affinity chromatography. The adhesive properties of purified recombinant Mgfp-5 were compared with the commercial extracted mussel adhesive Cell-Tak by investigating adhesion force using atomic force microscopy, material surface coating, and quartz crystal microbalance. Even though further macroscale assays are needed, these microscale assays showed that recombinant Mgfp-5 has significant adhesive ability and may be useful as a bioadhesive in medical or underwater environments.

* Corresponding author. Mailing address: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea. Phone: 82-54-279-2280. Fax: 82-54-279-5528. E-mail: hjcha{at}postech.ac.kr.

Applied and Environmental Microbiology, June 2004, p. 3352-3359, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3352-3359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, H., Scherer, N. F., Messersmith, P. B. (2006). Single-molecule mechanics of mussel adhesion. Proc. Natl. Acad. Sci. USA 103: 12999-13003 [Abstract] [Full Text]  


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