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Applied and Environmental Microbiology, June 2004, p. 3528-3534, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3528-3534.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Phylogenetic and Ecological Analysis of Novel Marine Stramenopiles

Department de Biologia Marina i Oceanografia, Institut de Ciències del Mar, CMIMA, CSIC, 08003 Barcelona,¹ Molecular Physiology and Biodiversity Department, Institut de Biologia Molecular de Barcelona, CSIC, 08034 Barcelona, Catalonia, Spain,² Station Biologique de Roscoff, 29682 Roscoff, France,³ Alfred Wegener Institute, D-27570 Bremerhaven, Germany⁴

Received 1 September 2003/ Accepted 25 January 2004

Culture-independent molecular analyses of open-sea microorganisms have revealed the existence and apparent abundance of novel eukaryotic lineages, opening new avenues for phylogenetic, evolutionary, and ecological research. Novel marine stramenopiles, identified by 18S ribosomal DNA sequences within the basal part of the stramenopile radiation but unrelated to any previously known group, constituted one of the most important novel lineages in these open-sea samples. Here we carry out a comparative analysis of novel stramenopiles, including new sequences from coastal genetic libraries presented here and sequences from recent reports from the open ocean and marine anoxic sites. Novel stramenopiles were found in all major habitats, generally accounting for a significant proportion of clones in genetic libraries. Phylogenetic analyses indicated the existence of 12 independent clusters. Some of these were restricted to anoxic or deep-sea environments, but the majority were typical components of coastal and open-sea waters. We specifically identified four clusters that were well represented in most marine surface waters (together they accounted for 74% of the novel stramenopile clones) and are the obvious targets for future research. Many sequences were retrieved from geographically distant regions, indicating that some organisms were cosmopolitan. Our study expands our knowledge on the phylogenetic diversity and distribution of novel marine stramenopiles and confirms that they are fundamental members of the marine eukaryotic picoplankton.

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