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Applied and Environmental Microbiology, June 2004, p. 3681-3686, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3681-3686.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Endogenous Xylose Pathway in Saccharomyces cerevisiae

Mervi H. Toivari,* Laura Salusjärvi, Laura Ruohonen, and Merja Penttilä

VTT Biotechnology, FIN-02044 VTT, Espoo, Finland

Received 12 September 2003/ Accepted 19 February 2004

The baker's yeast Saccharomyces cerevisiae is generally classified as a non-xylose-utilizing organism. We found that S. cerevisiae can grow on D-xylose when only the endogenous genes GRE3 (YHR104w), coding for a nonspecific aldose reductase, and XYL2 (YLR070c, ScXYL2), coding for a xylitol dehydrogenase (XDH), are overexpressed under endogenous promoters. In nontransformed S. cerevisiae strains, XDH activity was significantly higher in the presence of xylose, but xylose reductase (XR) activity was not affected by the choice of carbon source. The expression of SOR1, encoding a sorbitol dehydrogenase, was elevated in the presence of xylose as were the genes encoding transketolase and transaldolase. An S. cerevisiae strain carrying the XR and XDH enzymes from the xylose-utilizing yeast Pichia stipitis grew more quickly and accumulated less xylitol than did the strain overexpressing the endogenous enzymes. Overexpression of the GRE3 and ScXYL2 genes in the S. cerevisiae CEN.PK2 strain resulted in a growth rate of 0.01 g of cell dry mass liter–1 h–1 and a xylitol yield of 55% when xylose was the main carbon source.

* Corresponding author. Mailing address: VTT Biotechnology, P.O. Box 1500, FIN-02044 VTT, Finland. Phone: 358-9-456 7116. Fax: 358-9-455 2103. E-mail: Mervi.Toivari{at}vtt.fi.

Applied and Environmental Microbiology, June 2004, p. 3681-3686, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3681-3686.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Toivari, M. H., Ruohonen, L., Miasnikov, A. N., Richard, P., Penttila, M. (2007). Metabolic Engineering of Saccharomyces cerevisiae for Conversion of D-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols. Appl. Environ. Microbiol. 73: 5471-5476 [Abstract] [Full Text]  


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