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Applied and Environmental Microbiology, June 2004, p. 3745-3750, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3745-3750.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Noninvasive Pigment Identification in Single Cells from Living Phototrophic Biofilms by Confocal Imaging Spectrofluorometry

M. Roldán,1 F. Thomas,2 S. Castel,3 A. Quesada,4 and M. Hernández-Mariné1*

Departament de Productes Naturals, Biologia Vegetal i Edafologia,1 Serveis Cientificotècnics, Barcelona Science Park, Universitat de Barcelona,3 Institut de Robòtica i Informàtica Industrial (CSIC-UPC), E-08028 Barcelona,2 Departamento de Biología, Universidad Autónoma de Madrid, E-28049 Madrid, Spain4

Received 1 October 2003/ Accepted 20 February 2004

A new imaging technique for the analysis of fluorescent pigments from a single cell is reported. It is based on confocal scanning laser microscopy coupled with spectrofluorometric methods. The setup allows simultaneous establishment of the relationships among pigment analysis in vivo, morphology, and three-dimensional localization inside thick intact microbial assemblages.

* Corresponding author. Mailing address: Departament de Productes Naturals, Biologia Vegetal i Edafologia, Universitat de Barcelona, Av. Joan XXIII s/n, E-08028 Barcelona, Spain. Phone: (34) 93 4024490. Fax: (34) 93 4035879. E-mail: hernande{at}farmacia.far.ub.es.

Applied and Environmental Microbiology, June 2004, p. 3745-3750, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3745-3750.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Roldan, M., Oliva, F., Gonzalez del Valle, M. A., Saiz-Jimenez, C., Hernandez-Marine, M. (2006). Does Green Light Influence the Fluorescence Properties and Structure of Phototrophic Biofilms?. Appl. Environ. Microbiol. 72: 3026-3031 [Abstract] [Full Text]  


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