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Applied and Environmental Microbiology, June 2004, p. 3754-3757, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3754-3757.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diverse Tetracycline Resistance Genotypes of Megasphaera elsdenii Strains Selectively Cultured from Swine Feces

Thaddeus B. Stanton,1* Jennifer S. McDowall,2 and Mark A. Rasmussen1

Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010-0070,1 Division of Molecular Genetics, University of Glasgow, Glasgow G12 8QQ, United Kingdom2

Received 22 December 2003/ Accepted 26 February 2004

A total of 30 Megasphaera elsdenii strains, selectively isolated from the feces of organically raised swine by using Me109 M medium, and one bovine strain were analyzed for tetracycline resistance genotypic and phenotypic traits. Tetracycline-resistant strains carried tet(O), tet(W), or a tet gene mosaic of tet(O) and tet(W). M. elsdenii strains carrying tet(OWO) genes exhibited the highest tetracycline MICs (128 to >256 µg/ml), suggesting that tet(O)-tet(W) mosaic genes provide the selective advantage of greater tetracycline resistance for this species. Seven tet genotypes are now known for M. elsdenii, an archetype commensal anaerobe and model for tet gene evolution in the mammalian intestinal tract.

* Corresponding author. Mailing address: USDA Agriculture Research Service, National Animal Disease Center, 2300 N. Dayton Rd., P.O. Box 70, Ames, IA 50010-0070. Phone: (515) 663-7495. Fax: (515) 663-7458. E-mail: tstanton{at}nadc.ars.usda.gov.

Applied and Environmental Microbiology, June 2004, p. 3754-3757, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3754-3757.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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