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Applied and Environmental Microbiology, June 2004, p. 3761-3765, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3761-3765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Molecular and Biological Characterization of a Cryptosporidium molnari-Like Isolate from a Guppy (Poecilia reticulata)

Una Ryan,1* Amanda O'Hara,1 and Lihua Xiao2

Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia,1 Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 303412

Received 7 November 2003/ Accepted 23 February 2004

Histological, morphological, genetic, and phylogenetic analyses of a Cryptosporidium molnari-like isolate from a guppy (Poecilia reticulata) identified stages consistent with those of C. molnari and revealed that C. molnari is genetically very distinct from all other species of Cryptosporidium. This study represents the first genetic characterization of C. molnari.

* Corresponding author. Mailing address: Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. Phone: 61 8 9360 2482. Fax: 61 8 9310 4144. E-mail: unaryan{at}central.murdoch.edu.au.

Applied and Environmental Microbiology, June 2004, p. 3761-3765, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3761-3765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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