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Applied and Environmental Microbiology, June 2004, p. 3766-3768, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3766-3768.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Immobilization of Saccharomyces cerevisiae Cystathionine {gamma}-Lyase and Application of the Product to Cystathionine Synthesis

Shuzo Yamagata, Tsuyoshi Akamatsu, and Tomonori Iwama*

Department of Biotechnology, Faculty of Agriculture, Gifu University, Gifu 501-1193, Japan

Received 4 September 2003/ Accepted 23 February 2004

Cystathionine {gamma}-lyase of Saccharomyces cerevisiae was immobilized to aminohexyl-Sepharose through the cofactor pyridoxal 5'-phosphate and was characterized with respect to its cystathionine {gamma}-synthase activity. The immobilized product was so stable that it repeatedly catalyzed as many as five cycles of the reaction without losing activity.

* Corresponding author. Mailing address: Department of Applied Life Sciences, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan. Phone: 81-58-293-2932. Fax: 81-58-293-2932. E-mail: iwamatom{at}cc.gifu-u.ac.jp.

Applied and Environmental Microbiology, June 2004, p. 3766-3768, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3766-3768.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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